GABA-A receptor gamma2 224 003 品牌【Synaptic Systems】

Cat. No. 224 003

50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

Applications

WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 (see remarks) gallery  
IHC: 1 : 1000 (see remarks) gallery  
IHC-P: not tested yet
EM: yes

Immunogen

Synthetic peptide corresponding to AA 39 to 67 from mouse GABA-A receptor γ2 (UniProt Id: P22723)

Reactivity

Reacts with: human (P18507), rat (P18508), mouse (P22723), monkey, zebrafish.
Other species not tested yet.

Specificity

Specific for GABA-A receptor γ2. Does not discriminate between the L and S form. K.O. validated PubMed: 24849349

Matching control protein/peptide

224-0P

Remarks

WB:  GABA-A receptor gamma2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
ICC: This antibody is also suitable for the surface staining of living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents.
IHC: For best results use the protocol of Schneider Gasser et al., 2006.

Glyoxal fixation according to Konno et al. 2023 has improved signal quality and strength.