Synapsin 1-106008 品牌【Synaptic Systems】

Cat. No. 106 008

50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

Applications

WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery  
IP: yes
ICC: 1 : 1000 gallery  
IHC: 1 : 200 gallery  
IHC-P: 1 : 1000 gallery  
IHC-G: (see remarks) gallery  
ExM: yes (see remarks) gallery  
DNA-PAINT: yes (see remarks)

Clone

Rb46.1

Subtype

IgG1 (κ light chain)

Immunogen

full-length recombinant rat Synapsin1 (UniProt Id: P09951)

Epitop

Epitop: AA 435 to 475 from rat Synapsin1 (UniProt Id: P09951)

Reactivity

Reacts with: human (P17600), rat (P09951), mouse (O88935), mammals.
Weaker signal: chicken, zebrafish, other vertebrates.
Other species not tested yet.

Specificity

Specific for synapsin 1a and 1b independent of phosphorylation state. K.O. validated

Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 46.1. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IHC-G: Fixation with 9% glyoxal, 8% acetic acid, in ddH2O, pH 4.2-4.4, according to Konno et al. 2023 is recommended.
ExM: This antibody has been successfully used for the epitope-preserving magnified analysis of the proteome (eMAP) expansion microscopy method (Park et al. 2021. PMID: 34767453).
DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614).