Synaptophysin1 antibody - 101011 品牌【Synaptic Systems】

Cat. No. 101 011

50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

Applications

WB: 1 : 10000 (AP staining) gallery  
IP: yes
ICC: 1 : 100 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 500 up to 1 : 1000 gallery  
ExM: yes (see remarks) gallery  
EM: yes
ELISA: yes (see remarks)

Clone

7.2

Subtype

IgG1 (λ light chain)

Immunogen

Full-length recombinant rat Synaptophysin (UniProt Id: P07825)

Epitop

Epitop: AA 219 to 307 from rat Synaptophysin1 (UniProt Id: P07825)
corresponding to the cytoplasmic tail.

Reactivity

Reacts with: human (P08247), rat (P07825), mouse (Q62277), mammals.
Weaker signal: zebrafish, other vertebrates.
Other species not tested yet.

Specificity

K.O. validated PubMed: 31940485

Remarks

Widely used as marker for nerve terminals and neuroendocrine tumors. For unknown reason, neuronal synaptophysin is better recognised than neuroendocrine synaptophysin. If this is a problem, the polyclonal rabbit antibody, cat. no. 101 002 or 101 203 are recommended.
ExM: This antibody has been successfully used for the epitope-preserving magnified analysis of the proteome (eMAP) expansion microscopy method (Park et al. 2021. PMID: 34767453).
Variance from the standard protocol:
Expansion solution for imaging: 0.02X PBS
ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 101 002 as detector antibody. The ELISA-protocol for membrane proteins is recommended.